Mastering Tissue Dissociation – Episode 1: THE HEART
05 Aug 2024
The dissociation of mouse heart tissue into single cells is a crucial step in many biomedical investigations However, obtaining viable...
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Sample preparation is critical to master molecules extraction from biological samples. Bertin Health & Life Sciences offers efficient and flexible Precellys tissue homogenizers for grinding any samples prior to analysis.
In order to extract the molecules of interest (extraction of RNA, DNA, proteins, metabolites, etc.) from biological samples, sample preparation procedures generally include a tissue homogenization or cell disruption step.
The Precellys® homogenizers from Bertin Health & Life Sciences use bead-beating technology to homogenize biological samples, such as human, animal or plant tissues, or to lyse micro-organisms. In this way, the biological samples are ground via multi-directional movement (3D bead-beating), the speed of which can reach up to 10,000 rotations per minute, depending on the equipment. Bead-beating is the gold standard method for tissue homogenization compared to standard techniques such as: mortar & pestle, sonication, blender, high pressure homogenizer. It takes just a few dozen seconds to homogenize the samples, which are then ready for an analysis of the molecules of interest (nucleic acids, proteins, metabolites) using different analysis techniques: PCR, sequencing, Western Blot, liquid chromatography, etc. This represents a considerable time saving that simplifies sample preparation protocols.
The high-performance characteristics of the Bertin Health & Life Sciences homogenizers, together with a wide variety of lysing and grinding kits, offer excellent reproducibility and a great degree of flexibility in a broad range of research areas including microbiology, pharmacology, cancer, forensic medicine, the environment, and even agronomy.
The Precellys range offers reliable and cross-contamination-free sample preparation, ideal for routine applications and advanced research projects.
• Perfect for occasional use, the compact Minilys homogenizer processes up to three samples simultaneously. Its versatility (compatible with 0.5, 2 and 7mL tubes) and small footprint make it a practical choice for laboratories needing conventional sample preparation.
• Precellys 24 Touch is designed for intensive daily use. This robust homogenizer can process up to 24 biological samples in 2mL and 0.5mL tubes. It meets the stringent requirements of laboratories operating at biological safety level 3.
• For even greater versatility, Precellys Evolution Touch homogenizer supports up to 96 samples with six different tube sizes, from 0.3mL to 50mL. It ensures safe homogenization in compliance with biological safety level 3 standards. Pair it with the Cryolys Evolution cooling system to protect heat-sensitive samples during homogenization, ensuring high-quality results. This homogenizer is also enhanced by Precellys Connect, a smart application that simplifies your workflows and helps you get the most out of your homogenizers.
05 Aug 2024
The dissociation of mouse heart tissue into single cells is a crucial step in many biomedical investigations However, obtaining viable...
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