In order to extract the molecules of interest (extraction of RNA, DNA, proteins, metabolites, etc.) from biological samples, sample preparation procedures generally include a tissue homogenization or cell disruption step.
The Precellys® homogenizers from Bertin Health & Life Sciences use bead-beating technology to homogenize biological samples, such as human, animal or plant tissues, or to lyse micro-organisms. In this way, the biological samples are ground via multi-directional movement (3D bead-beating), the speed of which can reach up to 10,000 rotations per minute, depending on the equipment. Bead-beating is the gold standard method for tissue homogenization compared to standard techniques such as: mortar & pestle, sonication, blender, high pressure homogenizer. It takes just a few dozen seconds to homogenize the samples, which are then ready for an analysis of the molecules of interest (nucleic acids, proteins, metabolites) using different analysis techniques: PCR, sequencing, Western Blot, liquid chromatography, etc. This represents a considerable time saving that simplifies sample preparation protocols.
The high-performance characteristics of the Bertin Health & Life Sciences homogenizers, together with a wide variety of lysing and grinding kits, offer excellent reproducibility and a great degree of flexibility in a broad range of research areas including microbiology, pharmacology, cancer, forensic medicine, the environment, and even agronomy.